林火对长白山自然保护区风灾区土壤性质的影响

以2012年长白山国家级自然保护区西坡风灾区森林火灾为研究背景，采用常规土壤分析方法研究了不同火烧强度下土壤理化性质的变化情况．结果表明：火烧后土壤容重和土壤含水率随火烧强度的增强显著下降；0～6cm土壤pH，土壤有机质，土壤全C、全N、速效P、速效K质量分数经轻度和中度火烧后均呈显著增加趋势；在火烧强度因子的控制下，对各养分质量分数进行的偏相关分析显示，除土壤pH外，其他各养分间存在显著的相关性．     

Role of IFNLR1 gene in PRRSV infection of PAM cells

BackgroundInterferon lambda receptor 1 (IFNLR1) is a type II cytokine receptor that clings to interleukins IL-28A, IL29B, and IL-29 referred to as type III IFNs (IFN-λs). IFN-λs act through the JAK-STAT signaling pathway to exert antiviral effects related to preventing and curing an infection. Although the immune function of IFN-λs in virus invasion has been described, the molecular mechanism of IFNLR1 in that process is unclear.ObjectivesThe purpose of this study was to elucidate the role of IFNLR1 in the pathogenesis and treatment of porcine reproductive and respiratory syndrome virus (PRRSV).MethodsThe effects of IFNLR1 on the proliferation of porcine alveolar macrophages (PAMs) during PRRSV infection were investigated using interference and overexpression methods.ResultsIn this study, the expressions of the IFNLR1 gene in the liver, large intestine, small intestine, kidney, and lung tissues of Dapulian pigs were significantly higher than those in Landrace pigs. It was determined that porcine IFNLR1 overexpression suppresses PRRSV replication. The qRT-PCR results revealed that overexpression of IFNLR1 upregulated antiviral and IFN-stimulated genes. IFNLR1 overexpression inhibits the proliferation of PAMs and upregulation of p-STAT1. By contrast, knockdown of IFNLR1 expression promotes PAMs proliferation. The G0/G1 phase proportion in IFNLR1-overexpressing cells increased, and the opposite change was observed in IFNLR1-underexpressing cells. After inhibition of the JAK/STAT signaling pathway, the G2/M phase proportion in the IFNLR1-overexpressing cells showed a significant increasing trend. In conclusion, overexpression of IFNLR1 induces activation of the JAK/STAT pathway, thereby inhibiting the proliferation of PAMs infected with PRRSV.ConclusionExpression of the IFNLR1 gene has an important regulatory role in PRRSV-infected PAMs, indicating it has potential as a molecular target in developing a new strategy for the treatment of PRRSV.     

小白菜害虫优化防治技术研究

在害虫发生初期减少农药防治次数能明显降低害虫发生后期的防治难度，５月底前收获的小白菜宜防治１次，在害虫发生盛期种植的小白菜，宜防治２次。     

SINPV PK基因的部分序列分析

重组质粒ｐＰ１８ＰＨ３上含有ＳＩＮＰＶ的部分蛋白激酶基因。ＳＩＮＰＶ部分的蛋白激酶氨基酸序列与ＨａＮ－ＰＶ、ＨｚＮＰＶ、ＳｐｌｉＮＰＶ、ＡｆＮＰＶ、ＡｃＮＰＶ、ＬｄＮＰＶ蛋白激酶的氨基酸序列的同源性分别为４５％、８９％、３７％、４４％、３８％和３７％，其上含有蛋白激酶特征序列ＩＶＨＡＮＤＶＫＬＥＮＶＬ。     

大庆市粗饲料供应与利用的对策分析

通过调查和研究，分析了大庆市粗饲料的生产和供应能力，确定了影响大庆市粗饲料供应与利用的主要影响因素。在此基础上，提出了大庆市粗饲料供应和利用的对策和建议。     

叶面喷施氨基酸水溶肥料对大白菜产量和VC含量的影响

以北京新3号大白菜为试验材料，在大白菜包心期喷施不同浓度氨基酸水溶肥料，测定大白菜产量及VC含量。结果表明：叶面喷施含氨基酸水溶肥料，大白菜产量提高5.8 %~12.4 %、VC含量增加15.8 %~51.7 %。     

微波在线式粮食水分检测系统

微波检测是一种新的水分无损检测方法.为此,针对粮食水分检测的特点和要求,设计了基于微波的粮食水分在线式检测系统,实现了对粮食水分含量的实时和连续检测;重点介绍了微波水分检测原理、系统组成结构、数据采集及结果分析.     

农业开发项目环境影响因素及评价方法研究

目前,在农业开发项目建设中,要求在取得经济效益的同时更加注重社会效益和生态效益.本文阐述了农业开发项目对生态环境的各种影响因素,在此基础上,总结了传统农业开发项目环境影响评价的方法,提出了基于因子分析法和层次分析法的项目环境影响评价方法,以使农业开发项目的决策更加科学、合理.